Journal: Molecular Medicine Reports

Article Title: Alterations in the long non-coding RNA transcriptome in mesangial cells treated with aldosterone in vitro

doi: 10.3892/mmr.2017.7313

Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction quantification of microarray hybridization. (A) The relative expression level of lncRNAs BC168211, BC088254, AF336872, AY325162, BC168687, AF230638 and BC167085. (B) The relative expression levels of upregulated mRNAs, NM_001108598, NM_001109190 and NM_001101018, and (C) downregulated mRNAs, NM_019347, NM_177,962 and NM_0,011,08823. *P<0.05 vs. the control group. ALD, aldosterone; lncRNAs, long non-coding RNAs; MCs, mesangial cells.

Article Snippet: The cells were stored at −80°C and sent to the LncRNA Expression Microarray (Arraystar, Rockville, MD, USA).

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Microarray, Hybridization, Expressing, Control

Journal: Journal of Clinical Laboratory Analysis

Article Title: Super‐enhancer–associated long noncoding RNA RP11‐569A11.1 inhibited cell progression and metastasis by regulating IFIT2 in colorectal cancer

doi: 10.1002/jcla.23780

Figure Lengend Snippet: SE‐LncRNA microarray analysis of CRC tissues. A, The scatter plot displayed variation in SE‐LncRNA expression between group‐N (normal) and group‐T (CRC). B, The volcano plot represented the fold change values and P‐values of the microarray data. C, Hierarchical clustering revealed differentially expressed SE‐LncRNAs (fold change >2, p ‐value <0.05). Red and green colors represented upregulated and downregulated SE‐LncRNAs, respectively

Article Snippet: Given that the expression abundance of SE‐LncRNA at lower levels than that of mRNA, and RNA‐seq is not sensitive to low‐abundance SE‐LncRNAs, we conducted Arraystar human SE‐LncRNAs microarray to detect differentially expressed SE‐LncRNAs.

Techniques: Microarray, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: A novel antisense lncRNA NT5E promotes progression by modulating the expression of SYNCRIP and predicts a poor prognosis in pancreatic cancer

doi: 10.1111/jcmm.15718

Figure Lengend Snippet: LncNT5E is up‐regulated in pancreatic cancer (PC) tissues and cell lines. A, The heat map from our previous lncRNA microarray reflected the differentially expressed lncRNAs in PC and normal tissues. T represents PC tissue, and N represents normal pancreatic tissue. ENST00000421594 indicates lncNT5E. B, Relative expression of lncNT5E in 45 paired PC and adjacent normal tissues. LncNT5E expression from all tissues was normalized to 18S expression (ΔCT) and then compared with adjacent normal tissues and converted to the fold change (2 −ΔΔCT ). C, Relative expression of lncNT5E in different cell lines. Data are shown as fold change (2 −ΔΔCT ). * P < .05, ** P < .01, *** P < .001

Article Snippet: We used microarray detection (H1602063, Arraystar Human LncRNA 8 × 60 k v3.0 1‐color) to study lncRNAs in three pairs of PC and adjacent normal tissues.

Techniques: Microarray, Expressing

Journal: Molecular Genetics & Genomic Medicine

Article Title: Identification and functional annotation of metabolism‐associated lnc RNA s and their related protein‐coding genes in gastric cancer

doi: 10.1002/mgg3.427

Figure Lengend Snippet: The top 10 enriched GO terms of differently expressed genes. (a) The top 10 enriched GO terms of upregulated genes. (b) The top 10 enriched GO terms of downregulated genes. (c) Heatmap of differentially expressed genes in our metabolic microarray. The blue bar in the row represents protein‐coding genes, while the red bar in the row represents lnc RNA s

Article Snippet: The LncPathTM human metabolism pathway lncRNA microarray (Arraystar, Rockville, MD, USA) was used, which contains probes for both lncRNAs and mRNAs to simultaneously profile the expression of 965 lncRNAs and 458 protein‐coding gene targets related to the metabolic signaling pathway.

Techniques: Microarray